Fish skin gel blocking buffer

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Susan Murphy
Susan Murphy's picture
Fish skin gel blocking buffer

We recently changed our blocking buffer from BSA to Fish Skin Gel to reduce non-specific binding in our immunofluorescent staining.  We saw remarkable improvement so continued with it but now have a new problem in that many of the slides are drying out when we incubate overnight in the fridge. However, this is not a problem for the blocking step (1 h at rm temp) and the 2nd antibody (1.5 h at rm temp). Nor is it a problem when we block with BSA.  We have been using the Fish Skin Gel at 2% which is the highest dilution recommended so I have tested blank slides with lower concentrations and found that 2% seems to partially dissolve the barrier.  So my question is I would like to know of other's experiences with Fish Skin Gel as a blocking buffer for IHC/IF, what concentration is optimal for blocking and incubating with the antibody (I read recently some are using it at 0.2%.) and has anyone had a similar experience.Thank you for your help -Sue

Fraser Moss
Fraser Moss's picture
Supplier

From whom do you purchase the fish skin gel?Vendor and part number please?

Susan Murphy
Susan Murphy's picture
fish skin gel

Sigma: G7041