FTIR

4 posts / 0 new
Last post
SIVANANDAN NAMA...
SIVANANDAN NAMACHIVAYAM's picture
FTIR

HI i have tried FTIR for the rapid detection of microorganisms from food and clinical source.do anybody suggest that is this FTIR method really helps in thdiagnosis, shall we use this as diagnostic tool?. and also it will be more helpfull if more focussed on the anlysis of data

varsha
varsha's picture
Sivanandam, if you give us

Sivanandam, if you give us some examples of its use in microbe diagnostic and why it may or maynot be the best method, let us know. I am not sure of the answer but I looked up what FTIR is useful for and decided to share it with SS community here.
Fourier Transformation InfraRed is a spectroscopy method for identification of molecules in a sample. When a beam of IR encounters a sample, some of which is reflected and some is absorbed or transmitted though to the detector. The IR spectrum received at the detector can be analyzed and matched with the database of known molecules. It is often used for ground solids like wood, corn etc.
An infrared spectrum represents a fingerprint of a sample with absorption peaks which
correspond to the frequencies of vibrations between the bonds of the atoms making up the material.
Because each different material is a unique combination of atoms, no two compounds produce the
exact same infrared spectrum. Therefore, infrared spectroscopy can result in a positive identification
(qualitative analysis) of every different kind of material. In addition, the size of the peaks in the
spectrum is a direct indication of the amount of material present. With modern software algorithms,
infrared is an excellent tool for quantitative analysis.

mmrc.caltech.edu/FTIR/FTIRintro.pdf

SIVANANDAN NAMA...
SIVANANDAN NAMACHIVAYAM's picture
dear varsha,

dear varsha,
thanks for ur comment. i was working in FTIR as a part of my research work, I use ftir to detect microorganisms particularly from serum , urine samples. it can also be used to discriminate normal and cancer samples. and to detect bacteria in food samples even species level.
my problem is analysing the data. lots of work going on in FTIR.
 

varsha
varsha's picture
I do not use FTIR myself. I

I do not use FTIR myself. I would suggest that you go through some recently punlished papers on use of FTIR for microbial detectin and contact the authors for help. You could send me a PM message.
Varsha