Percentage of resolving gel concentration is greater than that of sticking gel . What is the advatages?
Resolving gel has higher concentration of acrylamide which enables it to resolve proteins according to molecular weight. While stacking gel has lower concentration and also lower pH in comparison to resolving gels. Both these factors help in stacking of proteins as a fine band and migrate almost at the same rate (Isotachophoresis). This in turn is dependent upon the retardation factor induced by charge differences in Cl, glycinate and proteins nound with SDS.