When I stain cancer tissue by ICC with my own monoclonal primary antibody, biotinylated secondary antibody, avidin/biotin complex, and DAB as substrate, I counterstain with hematoxylin.
I would next like to stain a blood smear using the same ICC method...is it possible to counterstain with both Wright-Giemsa and Myeloperoxidase??? If this is possible, at what point in my protocol should I perform these stains, and in what order? Currently, I stain with hematoxylin after the DAB step, followed by 1:10 glacial acetic acid, ammonia water, tap water, graded ethanol, and finally xylene. Would this work for the W-G and Myeloperoxidase stain? Thanks for your input!!!