Bacterial Genomes Mining

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wendy wilson
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Bacterial Genomes Mining

A starting point for application of target-based genome mining is the choice of a specific chemical class of a natural product, followed by mining of genomic DNA sequence for genes encoding products that specify biosynthesis of the corresponding molecular scaffold. Once the candidate targets are detected, different techniques (e.g. NMR, GC-MS, LC-MS) can be used to identify the secondary metabolite it specifies and to increase its production to a level allowing purification and biological testing. The expression of the candidate targets could be activated or enhanced in the host organism by genetic manipulation (e.g. CRISPR/Cas9 system) and/or by optimization of the growth conditions. Alternatively, it can be cloned and heterologously expressed in a well-characterized or engineered host. The goals of genomes mining services based on phenotype and uniqueness of the bacterial isolate are to produce new metabolites for drug discovery.