Inhibitor - activation?

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sharmakaushikp's picture
Inhibitor - activation?

In case of inhibitor experiments (Protein kinase inhibitors for the dissection of signal transduction pathways), how do we explain the fact when an inhibitor itself produces some changes (either up-regulation or down-regulation) different from the basal level/control.
for example, if a experiment consists of 4 groups- control C, treatment T, inhibitor I, inhibitor + treatment I+T, and if the inhibitor itself in this case produces changes different from the control, how do we explain this observation?

Fraser Moss
Fraser Moss's picture
This means that in the basal

This means that in the basal state, the activity of the target of the inhibitor is not 0 and that the inhibitor is changing the basal activity when compared to the no drug control condition