perkin elmer luminometer injector suck up the reagent problem

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ziseputi
ziseputi's picture
perkin elmer luminometer injector suck up the reagent problem

our lab bought a new luminometer from perkinelmer, I used the injector to add the reagent to do the dual luciferase assay, but I found 1.5 ml reagent for each (3ml in total for luciferase and renilla substrate) went to the waste bottle without doing assays, Is it normal?

Shubhangi
Shubhangi's picture
Hi

Hi
when you use an injector for substrate additon you can not avoid this "void" volume. This is the volume of the tubing. But you can retrieve this substrate if you purge it into collection box and not waste bin.
This saves substrate. I have gone through similar situation with some other luminometer. Just check if your instrument has the facility.