Chambered Slides Microscopy Problem

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fly1346
fly1346's picture
Chambered Slides Microscopy Problem

Hello guys,

Im growing C2C12 cells in the LabTek chambered slides, but seem to be having a problem when it comes to the compound light microscopy as Its difficult to locate cells, to check for myotube formation. Anyone have any ideas as to what I could do to make the microscopy possible?

heehawmcduff
heehawmcduff's picture
Hi there,

Hi there,
I have used Labtek chamber slides for light and direct fluorescence microscopy (unfortunately they don't work terribly well with confocal).  Are you using the glass or the plastic ones? 
Also, are you using an inverted microscope to look at the cells or are you mounting the cells under a coverslip and trying to look at them through the coverslip rather than the slide itself.
Last question is; are you absolutely sure your cells are attaching and growing properly on the slides?
Regards

fly1346
fly1346's picture
Thanks for the quick reply.

Thanks for the quick reply. Im pretty sure these are plastic slides, and no I'm not using an inverted microscope, its a regular compound light microscope, and yes I believe cells are attached to the surface, as I was able to get a slight/small view of them growing yesterday, but today I cant. They've been in Diffrentiation medium for about 4 days now.
Cheers

Arvind Singh Pundir
Arvind Singh Pundir's picture
try decreasing the intensity

try decreasing the intensity of light for your microscope hope it works, if that doesn't  work then to make sure the cells are there stain one of your slide with some general stain and confirm ..............

heehawmcduff
heehawmcduff's picture
That's a good suggestion -

That's a good suggestion - just go right back to your microscopy basics, low mag, light, maybe even getting a focus plane on another slide with fixed cells then immediately changing over to your cells.