Black vs white 96 well plates

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MsV
MsV's picture
Black vs white 96 well plates

Now I want no smart arse answers to this one. So I guess black absorbs light and white reflects it. I have an assay that uses luminescence so I use solid white plates to block cross talk between wells. i have a new assay that uses fluorescence and the protocol states to use black plates.
Would this work with white plates or is it something specific to do with black plates?

Arvind Singh Pundir
Arvind Singh Pundir's picture
Hi MSV,

Hi MSV,
the possible answere to your querry may be that Black Minimizes-- background in fluorescence reading, back scattered light , cross-talk
While the white plates can be used for fluorescent applications, background fluorescence will be much higher than in black plates. The white plates are recommended for scintillation and luminescent detection systems in which the reflective properties of the plate enhance the signal without producing any endogenous background signal.

Ivan Delgado
Ivan Delgado's picture
 

 
Hi MsV,
The short answer to your question is: you need to try it out. In my experience you never know if an assay will work better (or not at all) in a white versus black plate. If the assay already works in one kind of plate, you may want to stick to it. Yet, if you need to get it to work from scratch, and you have access to both plates (not too hard considering that vendors tend to be pretty good at giving you free samples), try them both and see which one works best. I've seen assays that worked in only one type of plate, and others than worked better in one versus the other (typically white, but that may be my bias from ELISA assays).
My two cents. Good luck.