96well plates for imaging

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schrl
schrl's picture
96well plates for imaging

I am looking for  96 well cell culture plates with reduced meniscus. We would like to use these plates for single cell cloning in an imaging system and have problems with the dark ring resulted by the shadow of the meniscus during the imaging: the clones inside the ring at the border of the well aren't not visible at all. Does anybody has any clues?

PhilM
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 1. Are you using the black

 1. Are you using the black 96 well plates with a transparent base? e.g. http://www.bdbiosciences.com/ptProduct.jsp?action=SELECT&form=formTree_catBean&prodId=652364&wcm_page=/cellculture/neuronalcells/productlist&wcm_title=Cell%20Culture%20-%20Neuronal%20Cells%20-%20Product%20List

2. Are you filling the wells to the brim with solution so that there is a convex meniscus across the top of the well instead of a concave meniscus inside the well walls?

schrl
schrl's picture
Hi PhilM,

Hi PhilM,

thank you for your answer.

We are actually using Greiner and Sarstedt 96 well transparent plates. Do you have better experience with black plates?

I only filled 200 µl because I work with living cells and thought that the gas exchange is too much reduced when the fluid cylinder is too high. Furthermore I thought that the contamination risk will increase when the fluid has contact with the lid.