I've transformed human PBMCs with a virus and plated in soft agar (agarose, not low melt).
Colonies grew and I pulled plugs with pipet tips, plating the plugs in media in a 96 well plate. I had to pipet up and down a lot, since the plugs frequently stuck to the inside of the pipet tip.
I'm not sure if my cells were able to escape the agarose. Since I want to grow up transformed colonies, how do I get them out of the soft agar and growing happily?
Dumb side question: I know you can use agarase to recover nucleic acids from agarose. Would that work or would it kill my cells?