Has anyone been Alvetex 3D culture scaffold

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shankaranarayanan.v
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Has anyone been Alvetex 3D culture scaffold

HI dear alls,

I am a new entrant to the world of 3d cultures. Has anyone been using the alvetex 3d culture scaffolds. If yes, how was your experience with it. Is a there a way you can image the live cells using phase contrast microscope for routine checks? and how do we the scaffolds for live cell confocal imaging?

Jason King
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 If you want to check whether

 If you want to check whether cells you've added to the Alvetex have attached, you can stain the cells with neutral red. This is good for checking the whole cell population by eye. As the scaffold is not transparent, you can't see much with phase contrast.

We seeded the scaffolds with stem cells then fixed with PFA and sectioned the membrane to assess 3D structure formation. This worked very well.

shankaranarayanan.v
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Hi parvoman,

Hi parvoman,

Thank you for your kind reply. But as per my understanding i we perform fixation, the cells would die. Is there a way for live cell imaging or correct me if i am wrong.

Thank you,

Regards
shankar