My ladder is 100bp.I have two primers to test out. Primer Y which amplifies up to a product of 200bp and primer Z which amplifies up to 150bp fragment.I'm not very sure of how this all works. What does this mean? I thought that the ladder has to be bigger than the size of the primer. How can you measure with 100bp ladder.
YesYou need to use a bigger ladder 1K will do it. https://www.neb.com/products/n3232-1-kb-dna-ladder