Hi..everybody, I work about insect mtDNA before, I have big problem,
My problem is, We make PCR insect mtDNA and sequenced, analyze and assemble it with program but we want to enter the www.ncbi.com our sequence, and we can't succesfull, This is my problem, If you would like to help about our problem, we'll be very pleasure.
My problem is below..
. Please resubmit your sequence(s) with relevant features such as:
- coding regions (CDS features), partial or complete, including
nucleotide spans and reading frame. Using this information,
our software will add the amino acid translations for you.
- structural RNAs such as rRNAs, tRNAs, misc_RNAs, with
- features which may describe your sequence, such as
repeat_regions, UTRs, promoters with nucleotide spans