EF-1 α or CMV promoter

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bsengez
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EF-1 α or CMV promoter

Which one (EF-1 α or CMV promoter) helps efficient transcription? Is there any idea?

Guy Sovak
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I have a very good

I have a very good transcription efficiency with CMV promoter. Currently I am using pFLAG-CMV-2 and it is working just great.
Guy

bsengez
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Has pFLAG-CMV-2 got only CMV

Has pFLAG-CMV-2 got only CMV promoter on it? Or any other promoter?

Guy Sovak
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Here is the vector that I
bsengez
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Thanks. I'm using pBudCE4.1.

Thanks. I'm using pBudCE4.1. Here is the link for it:
http://www.invitrogen.com/content/sfs/vectors/pbudce4_1_map.pdf
Most people got great results with CMV promoter, but there is few articles about EF-1 α promoter. So, I want to ask this question in this forum.

Guy Sovak
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I am very happy that you are

I am very happy that you are using this forum for your scientific questions. You can tell all your fellow scientist about this site, as many scientists we have such the possibbility to get answers to our posted questions.
I am glad that the answer that you got helped you in your research.
I will read more about pBudCE4.1. Fortunatly the vector that I am using is very good for my protein expression.
Can you tell me how big is your gene?
Guy

bsengez
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Thanks for these wonderful

Thanks for these wonderful words. I like sharing my ideas with other scientists, because we are all trying for the same thing I suppose: To get the best results in our experimental designs. A suggestion may help a lot, I believe in it. (By the way, sorry for my English. I hope you can understand me easily.)
And my research: I'm trying to clone two HIV proteins, they are about 400 bp, very small as you can realize, but they have important roles in HIV regulation. I choosed vector pBudCE4.1, because it is a mammalian expression vector, and I want to see the effects of these proteins on human cells. I cloned one of them yesterday, I hope I can get good results like you.
All the best,
Burcu

Guy Sovak
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Hi Burcu,

Hi Burcu,
That what we are trying to achieve here. To help scientist all over the world. Building a community without borders.
And we always like to help each other.
Tell me are you using any Tags?
Guy

bsengez
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Thanks Guy, it is good to be

Thanks Guy, it is good to be the part of this forum.
Are you able to read about pBudCE4.1?

Guy Sovak
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Yes I looked at the map. But

Yes I looked at the map. But I prefer the one that I am using now that have already an N-terminus Flag tag.
Guy