I have a basic question. I have a construct encoding a protein. I know DNA sequence of the protein but I don't know the sequence of the construct outside the protein encoding part. So I need to perform DNA sequencing outside the known DNA. The question is: How can I find if any standard DNA sequencing primers (like T3, T7 etc.) can bind the part of DNA sequence that I know? I would prefer to use standard primers instead of making them myself if I have a choice. Manual alignment of primers to DNA doesn't work because a have a list of app.