I am trying to observe trp flouresence in human hemoglobin, and am using lyophilized human hemoglobin to do so.
Problem is, I am unable to observe any Trp signal whatsoever. Controls (just Trp alone), show a nice peak at at around 420 nm when excited at 280, but the human hgb shows nothing.
Does anyone have any experience with this? Does human hgb need to be in the reduced from (Fe2+) in order to see the Trp signal?