electron transport chain

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Mohan's picture
electron transport chain

There are four complexes in ETC of inner mitochondrial membrane.They transfer elctrons upon the metabolism of a substrate like succinate, malate, glutamate etc.
I want to know how to measure the electrons generated from each copmlexes if(suppose)succinate is matabolized.
In my experiments where I measure the free radicals generated from each complexes, i fall into confusion when each coplexes show different transfer rate. Would also be gald if anyone has an idea on electron amplification at each coplexes which are placed vectorially.
Any seminal paper discussing these subjects, if available please send me.