i am working about plant superoxide dismutase assay in rice
i use NBT method for this work...
after reading by spectophotometer, the values are very low!
and in some conditions, changing in values are not normal
e.g. i expected that in 20µM enzyme the activity be more than 10, but in some conditions that was inversly!
can you please tell me, what is my mistake?
thank you for your attenttion