I'm working on the assay of ACCase activity from a weed. the method used was based on that described by Cocker et al.(1999, Pseticide biochemistry and physiology, 65,169-180). Unfortunately, the ACCase remained no activity. Is there anyone that had assayed this ACCase, that could supplied some advice on the assay? In the assay, is there something that should be paied special attention to? Any help would be appreciated. Thanks a lot!