Homogenization Issue

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Shaymaa Ahmed
Shaymaa  Ahmed's picture
Homogenization Issue

Hi everyone,
I'm estimating the Oxidative stree parameters (both enzymatic and non-enzymatic) in various oragns mainly liver and brain.
And was wondering would it make a difference if I done the homogenization at first in liquid nitrogen then used the homogenization buffer (as they are different for each parameter) than direct homogenization using the appropriate buffer.
I'm seeking and advice from those who worked before on Oxidative stress.