Hi all,
In the process of doing western blotting, you have - at some point - to prepare the homogenizing buffer to be used on the tissue sample you're studying.
I've read in the protocol that describes the recipe (of homogenizing buffer preparation) that it's also called "SDS reducing buffer".
Yet, you need to use SDS as an ingredient in the preparation of this buffer!
So how would that be?
The "reducing" does not refer to reducing SDS detergent. It refers to the reducing agent -usually mercaptoethanol or dithiotheritol - that is added to reduce disulfide bonds of proteins that otherwise stick together.
Hi,
I guess you might use SDS in your homogizing buffer ,if you are doing western blotting after your SDS-PAGE. Please correct me if am wrong.
Balu...
Thanks guys.
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