This doesn't make sense to me!

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medfreak
medfreak's picture
This doesn't make sense to me!

 Hi all,

In the process of doing western blotting, you have - at some point - to prepare the homogenizing buffer to be used on the tissue sample you're studying.

I've read in the protocol that describes the recipe (of homogenizing buffer preparation) that it's also called "SDS reducing buffer".

Yet, you need to use SDS as an ingredient in the preparation of this buffer!

So how would that be?

Chuck Miller
Chuck Miller's picture
 The "reducing" does not

 The "reducing" does not refer to reducing SDS detergent. It refers to the reducing agent -usually mercaptoethanol or dithiotheritol - that is added to reduce disulfide bonds of proteins that otherwise stick together. 

Add colour 2 ur life
Add colour 2 ur life's picture
Hi,

Hi,
I guess you might use SDS in your homogizing buffer ,if you are doing western blotting after your SDS-PAGE. Please correct me if am wrong.

Balu...

medfreak
medfreak's picture
 Thanks guys.

 Thanks guys.

You're right Add color 2 ur life.