Can I use acetic acid instead of phosphoric acid in a 'Bradford' reagent?

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D1977H
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Can I use acetic acid instead of phosphoric acid in a 'Bradford' reagent?

Hi all!

This is my first post and I'm hoping that someone can help me.  I want to make my own Bradford reagent for protein quantification.  I have found plenty of protocols and recipes (some from this site) on how to make the Bradford reagent, but almost all the protocols use phosphoric acid - which my lab appears not to have. We have, however, glacial acetic acid an I wondered if I could use that instead.   All the info' I've read on bradford assays alude to the fact that coomasie dye binds to protein when in an 'acidic' environment (I'm paraphrasing), so I wondered if glacial acetic acid would be a good replacement? I can't really see a problem in doing this, but as chemistry is not really my strong point I thought I'd ask poeple more qualified to answer.  

Thanking you in advance,

Dirk.