This is my first post and I'm hoping that someone can help me. I want to make my own Bradford reagent for protein quantification. I have found plenty of protocols and recipes (some from this site) on how to make the Bradford reagent, but almost all the protocols use phosphoric acid - which my lab appears not to have. We have, however, glacial acetic acid an I wondered if I could use that instead. All the info' I've read on bradford assays alude to the fact that coomasie dye binds to protein when in an 'acidic' environment (I'm paraphrasing), so I wondered if glacial acetic acid would be a good replacement? I can't really see a problem in doing this, but as chemistry is not really my strong point I thought I'd ask poeple more qualified to answer.
Thanking you in advance,