BY2 cell, cell cycle duration

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biochemsitry12
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BY2 cell, cell cycle duration

Hi, i have recently applied HU for 24 hours to arrest tobacco BY2 cells in the S phase. After the incubation with HU, i washed it away and placed it in a nutrient rich medium to allow cell cycle continuation. I have obtained histograms at intervals of 2,5,12 and 24 hrs. However, could someone explain to me how i could use the histograms to deduce the lenght of S-phase to G2/M phase ?