many replies possible:
- library intrinsically unable to bind your antigen
- too little antigen used
- too harsh washing steps
- stickiness of phage particles that bind tube surface with aspecific binding overweighing antigen-specific binding
- ... many more
sugg:
try panning on biotinylated antigen with elution by reduction (antigen biotinylated with a reducible biotinylating agent). usually elution is much more specific. try performing panning in glass tubes to decrease phage aspecific retention (I never tried this yet)
phage display can be very tricky. Before starting you should make sure that the library stock you are using is fresh and that it well represents the theoretical size of the library (n° of different clones). good luck
We are using the best i-phone and it displays working and it is really helpful for us. And if I have more problem then go to the technical support which provides the best support and it is very important for us. And In this i-phone, I can activate iphone without sim and it is working very fine.
many replies possible:
- library intrinsically unable to bind your antigen
- too little antigen used
- too harsh washing steps
- stickiness of phage particles that bind tube surface with aspecific binding overweighing antigen-specific binding
- ... many more
sugg:
try panning on biotinylated antigen with elution by reduction (antigen biotinylated with a reducible biotinylating agent). usually elution is much more specific. try performing panning in glass tubes to decrease phage aspecific retention (I never tried this yet)
phage display can be very tricky. Before starting you should make sure that the library stock you are using is fresh and that it well represents the theoretical size of the library (n° of different clones). good luck
We are using the best i-phone and it displays working and it is really helpful for us. And if I have more problem then go to the technical support which provides the best support and it is very important for us. And In this i-phone, I can activate iphone without sim and it is working very fine.