No enrichment from phage library.

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MicroKiller's picture
No enrichment from phage library.

Hi all.. i am a newbie working on phage display in my lab. I tried my luck in making a Fab format library on phage. I jst started panning and i have a scFv library together as a control for my selection process. It seems that i am getting enrichment for the scFv but not for the Fab library. I have done 4 rounds of selections on 10 different biotinylated proteins. Does anyone have any suggestions?? Its a brand new library which has not been tested so i am abit clueless.. I am planning to do a fab screening to see if any fab is actually presented on the phage.. and i am considering continuing the selection for another 2 rounds.. does anyone have any experience dealing with Fab libraries???