I'm doing perforate patch clamp with nystatin. I have a problem with my pipet resistance (R), the resitance in the bath should be around 15-25, but I have 45-60, I redid my pipet solution, sonicate nystatin in DMSO, and my pipet solution + nystatin in DMSO. I cheked the puller filament ! but I still have a higher pipet resistance. it is a nightmare !! Do you have please any suggestions!!
Thank you in advance for yout ansewers....