I am attempting to patch clamp SCN (an area of the hypothalamus) cells in the whole cell configuration. I have extensive experience with HEK cells but have only been patching these cells in a brain slice for the past few months with limited sucess.
These cells are small (8-12 um) GABA-ergic neurons not organized in a laminar structure like hioppocampus and my big problem is that once I get a good seal and go whole cell the access resistance is just too high. I have tried to improve access by reapplying negative pressure or zapping but with limited success.
Does anyone have any tips or tricks for improving access to the cell? I have tried different pipettes and different techniques for going whole cell but nothing is working for me.