CRISPR/Cas9-mediated ChIP

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wendy wilson
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CRISPR/Cas9-mediated ChIP

Chromatin immunoprecipitation (ChIP) assay is a powerful and versatile immunoprecipitation assay for detecting protein-DNA interactions in the natural chromatin environment of cells. The assay can be used to identify a variety of proteins associated with a particular region of the genome, such as a transcription factor at a promoter or other DNA binding site, and possibly a cis protein, or conversely, to identify a genome associated with a particular protein. In addition, ChIP assays can be used to define spatial and temporal relationships for specific protein-DNA interactions.