I was just wondering if anyone knows how much spike solution can be added to the sample in spiking/fortification? I've read somewhere that the amount of spike should not go over 10% of the total amount of sample you have. But the thing is, I cannot find the reference for this. I forgot where I read this. Also Thermo Scientific states that it is only 5%. So I was kind of wondering if you know the % amount for spiking or if you have other references that points to either 5%, 10% or others. It would be great if you can point me to the reference.
Thanks