Protein extraction from kidney tissue

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Harshal
Harshal's picture
Protein extraction from kidney tissue

 Hello everyone,

I want to run protein extracts from kidney tissue onto a column chormatography. i am just not sure what buffer to use to extract the protein from kidney tissue so that i get the proteins in the actual state...and not degrade it. any help is appreciated.

Thank you.
Harshal

Sami Tuomivaara
Sami Tuomivaara's picture
Harshal,

Harshal,

There are numerous buffers you could use, here's one protocol (Lee CM (2007) Meth Mol Biol 362). Also, you can go to Sigma, Pierce, and many more vendor websites, they have kits for this purpose.

Cheers,