please explain with any example for non aqeous reversphase chromatography.and how to convert method from normal phase hplc to non aqeous reversphase hplc
Why do you think this change is necessary? What samples are you trying to separate. These two techniques require different columns and mobile phase combinations, so conversions may not be easy.
can i use cyno column for both.i think useage of cyno column for the both normal phase and non aqeous reversphase hplc .
my problem is when i am separating my sample in rp-hplc it is converting in to n-1 stage (water sensitive ).therefore iwas developed a method in normal phase with cyno column.when i am separating in normal phase i was observed unknown peaks .to know that masses of the unkowns peaks.so i to develop a method non aqeous revers-phase for lc-ms submission .
my chromatographic conditions in normal phase
mobile phase :n-hexane:ipa:NH3(85:15:0.2 %v/v)
column :inertsil cyno ,250cm,4.6mm,5μ flow :0.8ml/min
Below are some examples of non-aqueous RP for lipids done on Cadenza CD-C18:
Usually such fat soluble solutes are separated by normal phase chromatography.
I agree with Dr. Analytical - trying to convert from NP to RP will be a huge headache
(elution order will be reversed).