non aqeous reversphase chromatography

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thirupathi
thirupathi's picture
non aqeous reversphase chromatography

please explain with any example for non aqeous reversphase chromatography.and how to convert method from normal phase hplc to non aqeous reversphase hplc

Dr. Analytical
Dr. Analytical's picture
Why do you think this change

Why do you think this change is necessary?  What samples are you trying to separate.  These two techniques require different columns and mobile phase combinations, so conversions may not be easy. 

thirupathi
thirupathi's picture
sir,

sir,
can i use cyno column for both.i think useage of cyno column for the both normal phase and non aqeous reversphase hplc .
sir,
my problem is when i am separating my sample in rp-hplc it is converting in to n-1 stage (water sensitive ).therefore iwas developed a method in normal phase with cyno column.when i am separating in normal phase i was observed unknown peaks .to know that masses of the unkowns peaks.so i to develop a method non aqeous revers-phase for lc-ms submission .
my chromatographic conditions in normal phase
mobile phase :n-hexane:ipa:NH3(85:15:0.2 %v/v)
column :inertsil cyno ,250cm,4.6mm,5μ flow :0.8ml/min

Bryan Evans
Bryan Evans's picture
Below are some examples of

Below are some examples of non-aqueous RP for lipids done on Cadenza CD-C18:
http://www.imtaktusa.com/site_media/files/technical_information/TI095E.pdf
http://www.imtaktusa.com/site_media/files/technical_information/TI038E.pdf

Usually such fat soluble solutes are separated by normal phase chromatography.
I agree with Dr. Analytical - trying to convert from NP to RP will be a huge headache
(elution order will be reversed).