i am research chemist in pharma industry.my problem is when i am using rp-hplc my product will convert in to n-1 stage(my product highly sensitive to water ).therfore i selectd normal phase hplc .my product is highly polar compounds.when i am using silica column with n-hexane:IPA:NH3(85:15:0.2% v/v) mobile phase .i observed more tailing .then i choosen cyno column now peak shape is good.
why i am selected NH3 is when i used Tri ethyl amine ,Di ethyl amine &n-butyl amine as a modifier the signal to noise ratio is more at 220 nm .
my questions are
1.can i use NH3 as a modifier.
2.can i use this method for LOD and LOQ establishment for my product