HPLC

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AbdulHalim
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HPLC

We want to separate tagged peptides 8-35 amino acid long. FITC will be use as flourophore. Which type of column will give better separation??
Also we are want to separate sucrose, mannitol, öactose and chlorinated sugar like, sucralose in urine. Which type of column is better for this kind of separation??

What kind of gaurd column should we use for sugar separation?