bradford assay recipe - acid alternative?

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D1977H's picture
bradford assay recipe - acid alternative?

I seen various recipes (mostly the same) for 'home-made' Bradford recipes. The majority require (?) phosphoric acid and our lab doesn't have that.  I just wondered if I could use another acid instead? Say, glacial acetic acid? Does anyone know if this would work or not, as I've seen most literature on bradford assays saying that the binding of coomassie blue G to protein depends on it being in an acidified environment. Is there a chemical reason for the phosphoric acid, specifically, or would another acid work just as well?  Very curious.