I am characterizing a cell line (CHO) for mRNA expression of 4 different genes.
I performed qRTPCR to quantify the mRNA levels using 4 different primers sets and also b-actin (reference gene) primers.
But I do not have any control to compare/relate with it, and also i hav not isolated these genes, so cannot perform a standard curve for absolute quantifications.
Is it possible to use qRTPCR for knowing the relative levels of these genes? if so How can it be done?
Otherwise which other quick technique will help me out here.