I am having a poblem in HPLC Peak separation. I'm currently testing on a AI :Glufosinate Monoammonium.
The method seems to be useful to quantify single AI as well as a formulation that contains 2 AI. However, we have a new formulation that contains 3AI. Therefore , using this method, one of the AI is eluted at the same retention time as Glufosintae RT. How can i solve this. Below are the following parameters:
Thermo Hypersil SAX (10µ)
0.1 mol/l of dihydrogen phosphate solution