I'm new at designing primers and am looking for some help. I'm trying to amplify a gene that is 13.3KB. I realize this is too large to accurately do a PCR to amplify the whole gene. There are multiple papers that cite primers they designed/used for this receptor. I've been able to locate the forward/sense primer sequence in the sequence I obtained off of NCBI, however i have NOT been able to locate any of the reverse sequences. Does anyone have suggestions as to what I am doing wrong that I can't find the reverse/antisense primer sequences that are published in the published NCBI sequence?