can someone provide me the reason for substituting either Asp, Glu, or gluconate in the intracellular solution for chloride? I have noticed that for TRPM2 recordings, no one uses any chloride in their intracellular, and i am confused as to why this is.
use of aspartate, glutamate or gluconate in the intracellular solution
28 January 2010
#1
uppies
use of aspartate, glutamate or gluconate in the intracellular solution
ions like gluconate, methanesulfonate, or aspartate (i've never heard of glutamate being used) are larger than chloride, with lower mobilities. hence they contribute less to the liquid junction potential. you are correcting for your LJP aren't you?
yeah, i am. I think the issue is primarily with potentially opening up chloride channels, particularly in oocytes, as there are Ca2+ activated chloride channels.