Can you help me with an ongoing discussion:
Do you use Mr. Frosty at room temperature or pre-cool it to 4º when preparing chilled samples before putting it into the freezer? Either way, why is that your preference?
Thanks, JES
Can you help me with an ongoing discussion:
Do you use Mr. Frosty at room temperature or pre-cool it to 4º when preparing chilled samples before putting it into the freezer? Either way, why is that your preference?
Thanks, JES
I actually keep my Mr Frosty at -20 so that the cells freeze very rapidly and are less susceptible to DMSO cytotoxicity. The cells then go straight into the -80 overnight then into liquid nitrogen or -150 the next day.
I guess it's personal preference.
I usually precool it to ~4dC, also add freezing medium at 4dC to minimize protease action. Following that, it goes to -80dC o/n (where the device supposedly works at the 'optimum' rate of 1dC/min temp reduction), before removal from Mr Frosty and transfer to lN2.
Hi I keep Mr Frosty at -20 for a day before using for cryopreservation purpose.
We just started trying this new product:
http://www.capitalbiosciences.com/product/info/coolcell-nbcs-136.html
Claims to require no pre-cooling, and requires no isopropanol.
You are talking about CoolCell, a new cell freezing container, that does require no alcohol, no maintenance and no pre-cooling. Check it out at http://tinyurl.com/yh8b69k
I always precooled my Misters Frosty (the plural) to 4C and added 0C freezing medium to the cells. Cells often sat on ice for a bit if I had a lot of tubes at the same time.
I've been following a number of these Mr. Frosty versus Isopropanol-free systems threads and the one disadvantage I can see with the latter is that they only have enough space for 12 cryovials. The Isopropanol containing Mr. Frosty products that I use have space for 18 but this is also not really enough.
So to any product developers out there - design a foam based cooler that hold 30 vials and costs under $90. We'd probably buy 10 immediately! Also, for ease of storage in -80 freezers, they would work better if they were square and not round as this wastes precious space.
I totally second that - and if they happen to fit in the width of a standard freezer rack/box so that they can be neatly sloted between racks.... freezer bliss ;)
i drive a hummer myself - space trumps environment - more hazard but more space - if they could just make one that is bigger and needs no gas and fits into my garage that stores a lot of things i havent used in 50 years