we are doing enzyme estimation in chorionic villi sample for various metabolic disorder diseases. At present we have culturing method(Ref.Methods in cell science. 2003 25(3-4).149-154 ) for processing CV tissue. But we want protocol for preparing CV sample without culturing it, a direct method. Pls let us know how to process CV tissue in enzyme activity estimation. Plz help.
chorionic villi tissue preparation for LSD enzyme estimation