Scientist Solutions: International Life Science Community By Scientists For Scientists
    
Home » Forums » Biochemistry » General Biochemistry Discussion » expression confused

Thanks to our sponsors who make this site possible
Search forums by keyword   or   Create a new topic
 Link To Us    Bookmark Us     Chinese

expression confused

 RSS Feed

Would you like to save this topic, event, protocol or job so you can find it again easily?

Just click the "Save to My Lab Drawer" link and the item will be saved in the My Lab Drawer section of your bench space.

Available to members only. Please log in or register for your free account now.

Close Window 
View Printable Version
jawad
Malaysia

Send PM
See Mini bio

Status: Frog Egg
Frog Egg
Topic Started by jawad
on 3/9/2009 2:10 AM   
Reply to this post Go to the top of the page

hi,


Actually i have problem withexpression of my two genes. The first one is 14kd, i used BL21 not Bl21 (DE3) as a expression host.  i got bands in 14kd and its confirm by western blott. The second gene is 24kd, i perform expression and i got very sharp bands in 14kd also like the first gene, when i do western blott i detect these bands in same size 14kd. So now i dont know why i got 14kd bands in second gene and if these bands (first and second gene) relative to mine.


I realy need help.


Thanks,


Laith
Replies
Monu
India

Send PM
See Mini bio

Status: Adult Frog
Adult Frog
Posted By Monu
on 3/28/2009 0:36 AM   
Reply to this post Go to the top of the page

Dear Jawad

Why dont you try with 2D gel elec? May be you r problem can be solved. I will ask my other colleagues also.

Science with Truth...
As a Scientist Solutions member, you are able to register a positive vote for any topic which you believe is useful and relevant to our board or any reply which you believe is especially well worded and helpful.

By participating in the voting, you will be helping to identify the best topics & replies on the board.

You may vote once for any one post, and you may not vote for your own posts.

A post (topic or reply) will earn one "thumbs up" icon for every 10 votes received (up to 3 thumbs up), and the person who made the post will also earn two bonus points.

learn more about member points.

Close Window 


Board Rules | advertise | Privacy

Copyright © 2004-2012 Scientist Solutions, All Rights Reserved.
Resources from NCBI used on this site.