Hi,
I'm trying to grow E. coli biofilms in a microtitre plate. I have done these using S. aureus strains before with Tryptic Soy Broth (TSB) + 3% NaCl + 0.5% Gluc. However, when I tried the same with E. coli, biofilm formation in strains that should have good growth did not.
Does anyone have a protocol/recipe for making the appropriate E. coli biofilm growth medium??
Thanks!