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Suitable medium?

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schwartz
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Topic Started by schwartz
on 1/12/2009 10:34 AM
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Hi,
I'm trying to grow E. coli biofilms in a microtitre plate. I have done these using S. aureus strains before with Tryptic Soy Broth (TSB) + 3% NaCl + 0.5% Gluc. However, when I tried the same with E. coli, biofilm formation in strains that should have good growth did not.

Does anyone have a protocol/recipe for making the appropriate E. coli biofilm growth medium??

Thanks!
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guy
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Posted By guy on 1/12/2009 13:35 PM
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Is it for a persistent infection research?
schwartz
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Posted By schwartz on 1/12/2009 14:12 PM
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Yes, you could say that. But I'm looking for a base medium that E. coli forms a good biofilm in. I know that different strains of E. coli might react differently to medium conditions, but I just need a widely used/accepted medium for this purpose.

A Timmer
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Posted By A Timmer on 1/12/2009 16:18 PM
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Here is a paper looking at various strains of E. coli in different media and biofilm formation:

Pubmed