Suitable medium? |
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Topic Started by schwartz
on 1/12/2009 10:34 AM
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Hi, I'm trying to grow E. coli biofilms in a microtitre plate. I have done these using S. aureus strains before with Tryptic Soy Broth (TSB) + 3% NaCl + 0.5% Gluc. However, when I tried the same with E. coli, biofilm formation in strains that should have good growth did not.
Does anyone have a protocol/recipe for making the appropriate E. coli biofilm growth medium??
Thanks!
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Replies
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Posted By guy
on 1/12/2009 13:35 PM
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Is it for a persistent infection research?
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Posted By schwartz
on 1/12/2009 14:12 PM
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Yes, you could say that. But I'm looking for a base medium that E. coli forms a good biofilm in. I know that different strains of E. coli might react differently to medium conditions, but I just need a widely used/accepted medium for this purpose.
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