Scientist Solutions: Life Science Discussions
 Refer A Friend    Link To Us    Bookmark Us       

English - Chinese             

Welcome to Scientist Solutions!

Cell Proliferation inhibitors

RSS Feed

pog
United States

Send PM
See Mini bio

Status: Frog Egg
Frog Egg



Topic Started by pog
on 1/12/2009 8:27 AM
Reply to this post Go to the top of the page

 Hi everybody!!, i'm doing angiogenic assays to test migration defects in my cells and between controls and ko cells the proliferation ratio is very different, i know that there are some inhibitors to block the proliferation and test the migration in 3D gels and transwell inserts but i can't find a good one. Please help me!!!


Replies
R Bishop
United States

Send PM
See Mini bio

Status: Moderator
Moderator



Posted By R Bishop on 1/12/2009 8:28 AM
Reply to this post Go to the top of the page
Hi poq,
Topic moved to Cell Proliferation Forum. hope you get some answers.

Rb
"Light will be thrown....."
-- Charles Darwin


guy
Canada

Send PM
See Mini bio

Status: Moderator
Moderator



Posted By guy on 1/12/2009 8:39 AM
Reply to this post Go to the top of the page
Try to read this paper :
http://circres.ahajournals.org/cgi/content/abstract/47/4/578


pog
United States

Send PM
See Mini bio

Status: Frog Egg
Frog Egg



Posted By pog on 1/12/2009 8:59 AM
Reply to this post Go to the top of the page
guy said:
Try to read this paper :
http://circres.ahajournals.org/cgi/content/abstract/47/4/578


i did it, i have to use heparin in my media, but the proliferation ratio remains different. I know that there are a drug just to add in the media, but i can't find it again.


Shubhangi
India

Send PM
See Mini bio

Status: Young Frog
Young Frog



Posted By Shubhangi on 3/31/2009 9:03 AM
Reply to this post Go to the top of the page

Hi


The simplest drug used by many researchers is Actinomycin D. It binds to DNA and stops replication. Cells will not divide but remain viable.



A Timmer
United States

Send PM
See Mini bio

Status: Moderator
Frog Laureate



Posted By A Timmer on 3/31/2009 9:48 AM
Reply to this post Go to the top of the page

You have to be careful using actinomycin D as it is a known inducer of apoptosis. Apoptosis can be induced with 1 ug/ml and will usually start to occur between 12-24 hours after actinomycin D treatment.



Shampa
India

Send PM
See Mini bio

Status: Adult Frog
Adult Frog



Posted By Shampa on 3/31/2009 11:10 AM
Reply to this post Go to the top of the page
Timmer is right as Actinomycin is avoided as its highly toxic and causes damage to genetic material. But still it is used in many labs as it is a useful tool in determining apoptosis and distinguishing between dead cells and live ones. (http://www.fermentek.co.il/7AAD.htm)

You can see more details:
http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/datasheet6/a1410dat.Par.0001.File.tmp/a1410dat.pdf
http://www.ias.ac.in/jarch/jbiosci/20/151-156.pdf


R Bishop
United States

Send PM
See Mini bio

Status: Moderator
Moderator



Posted By R Bishop on 3/31/2009 11:26 AM
Reply to this post Go to the top of the page

Poq,


What concentration of heparin are you using?  Heparin competes for growth factors required fro angiogenesis (FGF2, etc).  Are you adding serum as well?


 


Rus

"Light will be thrown....."
-- Charles Darwin


heehawmcduff
United States

Send PM
See Mini bio

Status: Frog Laureate
Frog Laureate



Posted By heehawmcduff on 4/9/2009 20:59 PM
Reply to this post Go to the top of the page

Doxycycline is also an effective inhibitor of cell proliferation but unfortunately at high doses you also run into the same problem i.e. induction of apoptosis.  I wonder if a low dose treatment could accomplish what you are looking for.


Regards