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Topic Started by pog
on 1/12/2009 8:27 AM
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Hi everybody!!, i'm doing angiogenic assays to test migration defects in my cells and between controls and ko cells the proliferation ratio is very different, i know that there are some inhibitors to block the proliferation and test the migration in 3D gels and transwell inserts but i can't find a good one. Please help me!!!

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Hi poq, Topic moved to Cell Proliferation Forum. hope you get some answers.
Rb
"Everything should be made as simple as possible, but no simpler." -- Einstein
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Posted By guy on 1/12/2009 8:39 AM
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Posted By pog on 1/12/2009 8:59 AM
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i did it, i have to use heparin in my media, but the proliferation ratio remains different. I know that there are a drug just to add in the media, but i can't find it again.
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Hi The simplest drug used by many researchers is Actinomycin D. It binds to DNA and stops replication. Cells will not divide but remain viable.
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You have to be careful using actinomycin D as it is a known inducer of apoptosis. Apoptosis can be induced with 1 ug/ml and will usually start to occur between 12-24 hours after actinomycin D treatment.
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Posted By R Bishop on 3/31/2009 11:26 AM
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Poq, What concentration of heparin are you using? Heparin competes for growth factors required fro angiogenesis (FGF2, etc). Are you adding serum as well? Rus
"Everything should be made as simple as possible, but no simpler." -- Einstein
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Doxycycline is also an effective inhibitor of cell proliferation but unfortunately at high doses you also run into the same problem i.e. induction of apoptosis. I wonder if a low dose treatment could accomplish what you are looking for. Regards
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Posted By kranthi on 10/24/2009 1:30 AM
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Hi,
can u tell me the simple procedure to find out DNA binding activity of anticancer drugs
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