Cell Proliferation inhibitors

 Hi everybody!!, i'm doing angiogenic assays to test migration defects in my cells and between controls and ko cells the proliferation ratio is very different, i know that there are some inhibitors to block the proliferation and test the migration in 3D gels and transwell inserts but i can't find a good one. Please help me!!!

Hi poq,
Topic moved to Cell Proliferation Forum. hope you get some answers.

Rb

Try to read this paper :
http://circres.ahajournals.org/cgi/content/abstract/47/4/578

Hi

The simplest drug used by many researchers is Actinomycin D. It binds to DNA and stops replication. Cells will not divide but remain viable.

You have to be careful using actinomycin D as it is a known inducer of apoptosis. Apoptosis can be induced with 1 ug/ml and will usually start to occur between 12-24 hours after actinomycin D treatment.

Timmer is right as Actinomycin is avoided as its highly toxic and causes damage to genetic material. But still it is used in many labs as it is a useful tool in determining apoptosis and distinguishing between dead cells and live ones. (http://www.fermentek.co.il/7AAD.htm)

You can see more details:
http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/datasheet6/a1410dat.Par.0001.File.tmp/a1410dat.pdf
http://www.ias.ac.in/jarch/jbiosci/20/151-156.pdf

Poq,

What concentration of heparin are you using?  Heparin competes for growth factors required fro angiogenesis (FGF2, etc).  Are you adding serum as well?

Rus

Doxycycline is also an effective inhibitor of cell proliferation but unfortunately at high doses you also run into the same problem i.e. induction of apoptosis.  I wonder if a low dose treatment could accomplish what you are looking for.

Regards