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Topic Started by pog
on 1/12/2009 8:27 AM
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Hi everybody!!, i'm doing angiogenic assays to test migration defects in my cells and between controls and ko cells the proliferation ratio is very different, i know that there are some inhibitors to block the proliferation and test the migration in 3D gels and transwell inserts but i can't find a good one. Please help me!!!
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Replies
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Hi poq, Topic moved to Cell Proliferation Forum. hope you get some answers. Rb
"Light will be thrown....."
-- Charles Darwin
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Posted By guy on 1/12/2009 8:39 AM
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Posted By pog on 1/12/2009 8:59 AM
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i did it, i have to use heparin in my media, but the proliferation ratio remains different. I know that there are a drug just to add in the media, but i can't find it again.
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Hi The simplest drug used by many researchers is Actinomycin D. It binds to DNA and stops replication. Cells will not divide but remain viable.
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You have to be careful using actinomycin D as it is a known inducer of apoptosis. Apoptosis can be induced with 1 ug/ml and will usually start to occur between 12-24 hours after actinomycin D treatment.
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Posted By R Bishop on 3/31/2009 11:26 AM
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Poq, What concentration of heparin are you using? Heparin competes for growth factors required fro angiogenesis (FGF2, etc). Are you adding serum as well? Rus
"Light will be thrown....."
-- Charles Darwin
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Doxycycline is also an effective inhibitor of cell proliferation but unfortunately at high doses you also run into the same problem i.e. induction of apoptosis. I wonder if a low dose treatment could accomplish what you are looking for. Regards
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