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Signal transduction assays

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Status: Froglet
Frog Laureate
Topic Started by roudi
on 5/1/2005 19:02 PM   
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Scintillation proximity assays (SPA) are commonly used for quantitative measurements of second messenger targets such as: cAMP, cGMP etc.

Can we use SPA to measure the mechanism of signal transduction? What are the radioisotopes of choice for such assays. We need to fund and start a project that involves signal transduction measurements and we are puzzled to find the best methods available.
Thanks


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abhishek
India

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Status: Tadpole
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Posted By abhishek
on 8/5/2009 0:40 AM   
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to get possible target you can use enhancement or blocker of target but to quantify radioisotopes can assist.



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