|Conventional hematotoxicity testing involves measuring different mature blood-cell components, over time after administation of an agent. For proliferating systems, major toxicity does not occur in mature cells but in the cells responsible for producing the mature cells, namely the stem and progenitor cells for the lymphohematopoeitic system.|
For stem cell hemotoxicity to play a role at all stages of drug development, the HALO ( Hematopoeitic/hematotoxicity Assays via Luminescence Output), was developed. Does anyone know the name of company/ies that provide Halo platforms?
HALO exactly stands for Hematopoietic/Hemocytotoxicity Assay
Output. HALO Tag fusion proteins
are used in different sub-cellular locations: Nucleus, Mitochondria
, Cytosol, etc. Cells
can be labeled with fluorescence
and images observed by fluorescent
microscopes. The HaloTag technology is available by Promega
www.promega.com and is a new method of labeling
within the cell
that can be used in functional assays
. It allows observing cells
over long period of time so studies such as drug
exposures can be done.