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LacZ/Immunohistochemistry

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Caty784

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 Send a personal messsage to Caty784 Reply with a quote from this post Go to the top of the page

Hi!
Please, can anyone explain the mechanism whereby the reporter gene, lacZ, is used in staining a piece of tissue for subsequent visualization of the product of the gene it is connected to? (I know that in the blue/white screening, it cleaves beta-gal and produces blue color, but how about in tissue itself???)
Thank you so much.
Cat

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 Posted Oct 03, 2008, 0:55 AM
samm

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 Send a personal messsage to samm Reply with a quote from this post Go to the top of the page

Check out the reply I posted earlier on to yourpost - I think this one is a repeat!

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Posted Oct 03, 2008, 15:36 PM
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