Hi!
Please, can anyone explain the mechanism whereby the reporter gene, lacZ, is used in staining a piece of tissue for subsequent visualization of the product of the gene it is connected to? (I know that in the blue/white screening, it cleaves beta-gal and produces blue color, but how about in tissue itself???)
Thank you so much.
Cat

Hi,
Maybe start with understanding reporter genes/proteins by reading this small page in this link.
http://www.bio.davidson.edu/courses/genomics/method/reporters.html
Please let me know if you need more exlanation.
Guy