Hi All,

I am a graduate student in Boston University. The project I am working on includes retrogradely label mitral cells in the mouse main olfactory bulb. I use CTb and it work well (tracer injection injected to amygdala). I also want distal dendrite being labeled. Because the dendrite is very long and the end of the dendrite is not well labeled. I wonder whether there is better way(different tracers/higher concentration) to back label distal dendrite.

Thank you very much !

Cam ( cammy@bu.edu)

Maybe Cobalt staining would help
Try this link for a paper about cobalt staining
http://www.springerlink.com/content/jg6k1rw7w4j7n8g5/fulltext.pdf?page=1
Guy