May I know about how many passages of HTP-1 cells that can be cultured? I have not started my research yet and I need to plan my research.

Thank you,

Sri
sri1227@yahoo.com

We usually avoid 'passaging' more than ~12-15 times or maintaining the cells more than ~5-6weeks. Since the cells are rather loosely adherent, all you need to do is use a 10-ml pipette to swish the medium up and down to detach - no trypsin is required. All you have to do is remove ~2/3rd the volume (use those cells for experiments), and replace with fresh medium.
If you are going to use them for transfections, ~10-12 days after thawing is a sweet spot.
Make a lot of frozen stocks early on.

These cells are fairly robust You should be just fine - so as long as you feed them regularly, handle them with the usual precautions to avoid contamination, and don't mix up constructs (yes, that has happened in the lab!).